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genome sequencing zymo fast quick rna viral kit zymo r1035  (Zymo Research)


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    Zymo Research genome sequencing zymo fast quick rna viral kit zymo r1035
    Genome Sequencing Zymo Fast Quick Rna Viral Kit Zymo R1035, supplied by Zymo Research, used in various techniques. Bioz Stars score: 96/100, based on 1165 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/genome sequencing zymo fast quick rna viral kit zymo r1035/product/Zymo Research
    Average 96 stars, based on 1165 article reviews
    genome sequencing zymo fast quick rna viral kit zymo r1035 - by Bioz Stars, 2026-03
    96/100 stars

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    A. S. aureus Newman, A. baumannii ATCC 19606, and P. aeruginosa PA14 were stained with DiSC3(5), treated with either 1×MIC of Bald’s eyesalve (purple) or water (black), and their fluorescence monitored for 1 h. There was increased DiSC3(5) fluorescence, indicating membrane depolarisation in samples treated with Bald’s eyesalve. The red arrow indicate point of treatment. Each line represents the mean of three technical replicates. B. S. aureus Newman, A. baumannii ATCC 19606, and P. aeruginosa PA14 were treated with 1×MIC of Bald’s eyesalve (purple) or water (grey) for 1 h, stained with propidium iodide, and fluorescence was measured after 1 h. Each symbol represents an individual bacterial culture; box plots show the fluorescence intensity (in RFU) of propidium iodide. C. S. aureus Newman and A. baumannii <t>ATCC</t> <t>19606</t> were treated with 2×MIC of Bald’s eyesalve or water for 2 h, stained with FM4-64, and then imaged with a widefield fluorescence microscope at oil immersion lens (x100) magnification. Increased FM4-64 fluorescence in S. aureus and the presence of dense spots of fluorescence in A. baumannii suggest membrane perturbation.
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    A. S. aureus Newman, A. baumannii ATCC 19606, and P. aeruginosa PA14 were stained with DiSC3(5), treated with either 1×MIC of Bald’s eyesalve (purple) or water (black), and their fluorescence monitored for 1 h. There was increased DiSC3(5) fluorescence, indicating membrane depolarisation in samples treated with Bald’s eyesalve. The red arrow indicate point of treatment. Each line represents the mean of three technical replicates. B. S. aureus Newman, A. baumannii ATCC 19606, and P. aeruginosa PA14 were treated with 1×MIC of Bald’s eyesalve (purple) or water (grey) for 1 h, stained with propidium iodide, and fluorescence was measured after 1 h. Each symbol represents an individual bacterial culture; box plots show the fluorescence intensity (in RFU) of propidium iodide. C. S. aureus Newman and A. baumannii ATCC 19606 were treated with 2×MIC of Bald’s eyesalve or water for 2 h, stained with FM4-64, and then imaged with a widefield fluorescence microscope at oil immersion lens (x100) magnification. Increased FM4-64 fluorescence in S. aureus and the presence of dense spots of fluorescence in A. baumannii suggest membrane perturbation.

    Journal: bioRxiv

    Article Title: Uncovering the multifaceted mechanism of action of a historical antimicrobial

    doi: 10.64898/2026.02.02.703249

    Figure Lengend Snippet: A. S. aureus Newman, A. baumannii ATCC 19606, and P. aeruginosa PA14 were stained with DiSC3(5), treated with either 1×MIC of Bald’s eyesalve (purple) or water (black), and their fluorescence monitored for 1 h. There was increased DiSC3(5) fluorescence, indicating membrane depolarisation in samples treated with Bald’s eyesalve. The red arrow indicate point of treatment. Each line represents the mean of three technical replicates. B. S. aureus Newman, A. baumannii ATCC 19606, and P. aeruginosa PA14 were treated with 1×MIC of Bald’s eyesalve (purple) or water (grey) for 1 h, stained with propidium iodide, and fluorescence was measured after 1 h. Each symbol represents an individual bacterial culture; box plots show the fluorescence intensity (in RFU) of propidium iodide. C. S. aureus Newman and A. baumannii ATCC 19606 were treated with 2×MIC of Bald’s eyesalve or water for 2 h, stained with FM4-64, and then imaged with a widefield fluorescence microscope at oil immersion lens (x100) magnification. Increased FM4-64 fluorescence in S. aureus and the presence of dense spots of fluorescence in A. baumannii suggest membrane perturbation.

    Article Snippet: For S. aureus , all reads were aligned to S. aureus Newman whole genome sequence (accession number - NC009641.1 , assembly - GCF_000010465.1), while A. baumannii reads were aligned to A. baumannii ATCC 19606 whole genome sequence (accession number - NZ_CP046654.1, assembly - GCF_009759685.1).

    Techniques: Staining, Fluorescence, Membrane, Microscopy

    Both A. baumannii ATCC 19606 and S. aureus Newman were treated with 2×MIC of Bald’s eyesalve or water for 2 h and imaged with a scanning electron microscope.

    Journal: bioRxiv

    Article Title: Uncovering the multifaceted mechanism of action of a historical antimicrobial

    doi: 10.64898/2026.02.02.703249

    Figure Lengend Snippet: Both A. baumannii ATCC 19606 and S. aureus Newman were treated with 2×MIC of Bald’s eyesalve or water for 2 h and imaged with a scanning electron microscope.

    Article Snippet: For S. aureus , all reads were aligned to S. aureus Newman whole genome sequence (accession number - NC009641.1 , assembly - GCF_000010465.1), while A. baumannii reads were aligned to A. baumannii ATCC 19606 whole genome sequence (accession number - NZ_CP046654.1, assembly - GCF_009759685.1).

    Techniques: Microscopy

    Significantly differentially expressed genes were defined as those with p-values ≤ 0.05 and |log2foldchange| ≥1.5 in treated vs. untreated samples; n = 3 and 4 cultures per treatment for A. baumannii ATCC 19606 and S. aureus Newman, respectively.

    Journal: bioRxiv

    Article Title: Uncovering the multifaceted mechanism of action of a historical antimicrobial

    doi: 10.64898/2026.02.02.703249

    Figure Lengend Snippet: Significantly differentially expressed genes were defined as those with p-values ≤ 0.05 and |log2foldchange| ≥1.5 in treated vs. untreated samples; n = 3 and 4 cultures per treatment for A. baumannii ATCC 19606 and S. aureus Newman, respectively.

    Article Snippet: For S. aureus , all reads were aligned to S. aureus Newman whole genome sequence (accession number - NC009641.1 , assembly - GCF_000010465.1), while A. baumannii reads were aligned to A. baumannii ATCC 19606 whole genome sequence (accession number - NZ_CP046654.1, assembly - GCF_009759685.1).

    Techniques:

    A. Expression profile of significantly differentially expressed (p < 0.05) biofilm-associated genes in S. aureus Newman in response to Bald’s eyesalve. Red dashed lines indicate |log2-fold change| = 1.5, n = 4 cultures per treatment. B. Expression profile of significantly differentially expressed (p < 0.05) biofilm-associated genes in A. baumannii ATCC 19606 in response to Bald’s eyesalve. Red dashed line indicates log2-fold change = - 1.5, n = 3 cultures per treatment.

    Journal: bioRxiv

    Article Title: Uncovering the multifaceted mechanism of action of a historical antimicrobial

    doi: 10.64898/2026.02.02.703249

    Figure Lengend Snippet: A. Expression profile of significantly differentially expressed (p < 0.05) biofilm-associated genes in S. aureus Newman in response to Bald’s eyesalve. Red dashed lines indicate |log2-fold change| = 1.5, n = 4 cultures per treatment. B. Expression profile of significantly differentially expressed (p < 0.05) biofilm-associated genes in A. baumannii ATCC 19606 in response to Bald’s eyesalve. Red dashed line indicates log2-fold change = - 1.5, n = 3 cultures per treatment.

    Article Snippet: For S. aureus , all reads were aligned to S. aureus Newman whole genome sequence (accession number - NC009641.1 , assembly - GCF_000010465.1), while A. baumannii reads were aligned to A. baumannii ATCC 19606 whole genome sequence (accession number - NZ_CP046654.1, assembly - GCF_009759685.1).

    Techniques: Expressing

    S. aureus Newman, A. baumannii ATCC 19606, and P. aeruginosa PA14 were subjected to subinhibitory concentrations of Bald’s eyesalve or antibiotic and subsequently passaged in different concentrations based on the previous day’s highest concentration with growth. This was done for 14 days. The graph’s axes show the highest of two tested concentrations in which cultures grew at each time point, expressed as a multiple of the ancestral MIC.

    Journal: bioRxiv

    Article Title: Uncovering the multifaceted mechanism of action of a historical antimicrobial

    doi: 10.64898/2026.02.02.703249

    Figure Lengend Snippet: S. aureus Newman, A. baumannii ATCC 19606, and P. aeruginosa PA14 were subjected to subinhibitory concentrations of Bald’s eyesalve or antibiotic and subsequently passaged in different concentrations based on the previous day’s highest concentration with growth. This was done for 14 days. The graph’s axes show the highest of two tested concentrations in which cultures grew at each time point, expressed as a multiple of the ancestral MIC.

    Article Snippet: For S. aureus , all reads were aligned to S. aureus Newman whole genome sequence (accession number - NC009641.1 , assembly - GCF_000010465.1), while A. baumannii reads were aligned to A. baumannii ATCC 19606 whole genome sequence (accession number - NZ_CP046654.1, assembly - GCF_009759685.1).

    Techniques: Concentration Assay